Proline accumulation has been shown to correlate with tolerance to drought and salt stresses in plants. Our goal was to compare the growth rate of transgenic rice plants in which the expression of a mothbean Δ1-pyrroline-5-carboxylate synthetase (p5cs) cDNA was driven separately with a constitutive and a stress-inducible promoter. We found that both constitutive expression and stress-inducible expression of the p5cs cDNA in transgenic rice have led to the accumulation of p5cs mRNA and proline. Third-generation (R2) transgenic rice seedlings showed significantly higher tolerance to stress produced by high levels of NaCl or water deficiency as judged by faster growth of shoots and roots in comparison with non-transformed plants. However, stress-inducible expression of the P5CS transgene showed significant advantages over the constitutive expression in increasing the biomass production of transgenic rice grown in soil under stress conditions.
Source: Plant Science (2004) Vol. 166, p. 941-948
Source: Plant Science (2004) Vol. 166, p. 941-948
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ReplyDeleteOptional Inducible Lentiviral System | GenTarget Inc - The same lentivector can be used for constitutive expression or optionally for the tetracycline inducible expression when a repressor, TetR present in advance.